| Gotthold Ephraim Lessing |
– Playwright |
| Jens Cronborg |
– Actor (Grev Appiani) |
| Carl Frederik Gottfried Dahlberg |
– Actor (Marinelli, Gonzagas kammerherre) |
| Thora Amalie Holm |
– Actor (Grevinne Orsina) |
| Johan Frederik Martens |
– Actor (Hector Gonzaga, prins av Guastaua) |
| Henriette Olsen |
– Actor (Emilie Galotti) |
| Julius Olsen |
– Actor (Conti, en maler / Angelo, bandit) |
| Nancy Simonsen |
– Actor (Claudia Galotti, Emilies mor) |
| Niels Christian Simonsen |
– Actor (Pirro, tjener) |
| Ditlev Staal |
– Actor (Odoardo Galotti, Emilies far) |
| Herr Svendsen - Julius Olsens teatertrupp |
– Actor (Babtista, tjener) |
| August Wandell |
– Actor (Camillo Rota) |
